Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: Enhanced production of recombinant proteins with Corynebacterium glutamicum by deletion of insertion sequences (IS elements)

Fig. 4

Comparison of GFP production in IS element-deleted mutants. a Analysis of fluorescence intensity in the cell by FACS. C. glutamicum WJ004 harboring pCES-H36-GFP and C. glutamicum WJ008 harboring pCES-H36-GFP are represented by squares and circles, respectively. Open triangles represent wild type C. glutamicum harboring pCES-H36-GFP. b Analysis of GFP production by Western blotting. Lanes 1, 2, and 3 represent the protein sample from wild type C. glutamicum, WJ004, and WJ008 harboring pCES-H36-GFP at 18 h. Lanes T and S represent total protein fraction and soluble protein fraction. Protein sample was prepared from the same cell concentration which was normalized to OD600 of 4

Back to article page