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Table 1 List of bacterial strains, plasmids used in this study

From: Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum

Strain or plasmids

Relevant characteristics

References

Strains

  

 E. coli XL1-Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacI q ZDM15 Tn10 (Tet r )]

Stratagenea

 L. citreum CB2567

Wild type

[39]

 L. amylovorus

Wild type

KCTC 3597

Plasmids

  

 pJH11

GST-fused PA domain 4, Ampr

[31]

 pCB4170

E. coli-L. citreum shuttle vector

[14]

 pCB4270

pCB4170 mutant

This study

 pCB4170-sfGFP

pCB4170 derivative, P710, sfGFP

This study

 pCB4170-GST

pCB4170 derivative, P710, GST

This study

 pCB4170-Amy

pCB4170 derivative, P710, α-amylase

This study

 pCB4270-sfGFP

pCB4270 derivative, P710, sfGFP

This study

 pCB4270-GST

pCB4270 derivative, P710, GST

This study

 pCB4270-Amy

pCB4270 derivative, P710, α-amylase

This study

  1. aStratagene (La Jolla, CA, USA)