Skip to main content
Fig. 6 | Microbial Cell Factories

Fig. 6

From: Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum

Fig. 6

Production of GST and α-amylase in L. citreum CB2567 harboring pCB4270-GST or pCB4270-Amy. a Western blot analysis of total and soluble fractions of L. citreum. Lanes 1 and 2, pCB4170; lanes 3 and 4, pCB4170-GST; lanes 5 and 6, pCB4270-GST; lanes 1, 3 and 5, total protein fractions; lanes 2, 4 and 6, soluble protein fraction. The arrowhead indicates GST protein b Detection of α-amylase activity on 0.5 % starch MRS-chloramphenicol plate. After incubation, the halos on the plate were visualized by staining with iodine solution. Spots 1 to 4, L. citreum CB2567 harboring pCB4170, pCB4170-Amy, pCB4270 and pCB4270-Amy, respectively. c Quantitative assay of α-amylase activity using EnzChek® Ultra Amylase Assay Kit. All experiments were done in triplicate

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us