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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum

Fig. 4

Determination of mutation point by sequence analysis. a The 1587 bp region containing two ORFs was randomized and point mutation was found at 4690 bp position which is located in inverted repeat between two ORFs. b Analysis of sfGFP expression by SDS-PAGE. Lane M, protein size marker (kDa); lane 1 pCB4170; lane 2 pCB4170-sfGFP, lane 3 pCB4270-sfGFP; lane 4 pCB4170-4690T-sfGFP. The arrowhead indicates the sfGFP

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Microbial Cell Factories

ISSN: 1475-2859

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