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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum

Fig. 3

Analysis of plasmid copy number in L. citreum. a Agarose gel electrophoresis of plasmids. After preparation of plasmids from L. citreum, all plasmids were digested by BamHI for linearization of plasmids. Lane M, DNA size marker (kb), lane 1, pCB4170; lane 2, pCB4270; lane 3, pCB4170-sfGFP; lane 4 pCB4270-sfGFP. The open and closed arrowheads indicate the pCB4270 and pCB4270-sfGFP, respectively. b Relative copy number value of pCB4170, pCB4270, pCB4170-sfGFP and pCB4270-sfGFP determined by quantitative PCR method

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Microbial Cell Factories

ISSN: 1475-2859

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