Fig. 2
Analysis of sfGFP expression in L. citreum by a SDS-PAGE and b western blotting. Protein samples (total and soluble protein fractions) were taken at 8Â h cultivation. Lane M, molecular weight markers (kDa); lanes 1 and 2, pCB4170; lanes 3 and 4, pCB4170-sfGFP; lanes 5 and 6, pCB4270-sfGFP; lanes 1, 3 and 5, total protein fractions; lanes 2, 4 and 6, soluble protein fraction. The arrowheads indicate the band of sfGFP. c The histogram of FACS analysis. pCB4170, pCB4170-sfGFP and pCB4270-sfGFP are represented in histogram by red, blue and green, respectively. M values indicate the mean fluorescent intensity