Fig. 1From: Development of a high-copy plasmid for enhanced production of recombinant proteins in Leuconostoc citreum Library construction and FACS screening. a Schematic diagram of plasmid pCB4170-sfGFP. Library was constructed by the randomization of the 32-region (1587 bp) related to the plasmid replication. b The histogram of FACS screening. L. citreum harboring the original pCB4170-sfGFP (green curve) was used as a control. The original library, 1st round sorted library and 2nd round sorted library are indicated in histogram by red, blue and orange, respectivelyBack to article page