Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis

Table 2 Purification of GFP protein using the ACE system

Step	Total protein (µg)	Protein (µg ml⁻¹)	Total activity (U mg⁻¹)	Protein yield (%)^a
Lysate	63,927	15,981	80
Flow through	53,463	13,365	0
Wash 1	3244	811	0
Wash 2	4220	1055	0
Elution 1	3049	6098	0
Elution 2	235	471	171	47
Elution 3	55	111	0
Elution 4	488	977	0

^aRecovery of fluorescence activity relative to the total activity of the soluble extract

ISSN: 1475-2859