Table 1 Oligonucleotides used in this study
Primer | Function | Sequence (5′ to 3′) |
|---|---|---|
mutf | Mutation of pAGDIcre1 (F) | CACACACACGAATTCTTGGAGTGGGAAGTCAAATAACTATTT |
mutr | Mutation of pAGDIcre1 (R) | CACACACACGAATTCTTCAGTATAACAAGGTTGATTTCT |
mutf1 | Mutation of pAGDIcre2 (F) | CACACACACGAATTCCCAAGTGGGAAGTCAAATAACTATTT |
mutr1 | Mutation of pAGDIcre2 (R) | CACACACACGAATTCTTTCAGTATAACAAGGTTGATTTCTT |
mutf4 | Mutation of pAGDIcre3 (F) | CACACACACGAATTCGTTCCCAAGTGGGAAGTCAAATAAC |
mutr4 | Mutation pAGDIcre3 (R) | CACACACACGAATTCAGTATAACAAGGTTGATTTCTTAAAAC |
prolF | Cloning of pep (F) | CACACACACCCATGGCTTATCCAGCTACACGTGC |
prolR | Cloning of pep (R) | CACACACACTCTAGATTAACGTCCTTGTGCAGC |
AgurBamHI | Cloning P aguR -AguR-P aguB cassette (F) | CCCCCCGGATCCGACAAGTTTGGCTCAGATTGCTTG |
PtcNco | Cloning P aguR -AguR-P aguB cassette (R) | CCCCCCATGGTGTTTATTCCTCCTGAATAAAATAG |
Expvfor1 | Insertion of His-Tag (F) | CACACACACCCATGGCTAATCGACTGCAGGAAAATTTATACTTCCAAGGTC |
Expvrev1 | Insertion of His-Tag (R) | CTATCAATCAAAGCAACACGTG |
GfF1 | Cloning of gfp (F) | CACACACACCCATGGAATTCAGTAAGGGAGAAGAACTTTTC |
GfR1 | Cloning of gfp (R) | CACACACACCTGCAGACTAGTTTTGTAGAGCTCATCCATGC |