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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis

Fig. 5

a Genetic map of the pACE expression vector. repC and repA, replication genes; cat, chloramphenicol resistance gene; aguR, gene encoding the regulatory agmatine-sensor-regulator protein AguR; P aguR , aguR promoter; RBS, ribosome binding site; T1, T2 and T3, transcription terminators (ΔG = −10.3, −9.7 and −8.3 kcal/mol respectively); P aguB*, aguB agmatine-inducible promoter carrying the A > T mutation in the fifth nucleotide of the cre site; MCS, multicloning site; HisTag; C-terminal histidine tag. Representative restriction sites are indicated. b Overview of the AguR/PaguB expression system including its components and role. AguR is the transmembrane agmatine (black filled circles) sensor and response regulator that activates P aguB , thereby triggering the massive overproduction of the recombinant target protein

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Microbial Cell Factories

ISSN: 1475-2859

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