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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Reversing methanogenesis to capture methane for liquid biofuel precursors

Fig. 4

Extracellular production of acetate from 13CH4 in ANME-1 Mcr-producing M. acetivorans via GC/MS and NMR. a and b show GC/MS spectra of culture supernatants used to identify acetate from 13CH4. Unlabeled acetate (12C Acetate, black arrows) has a molecular mass of 60, whereas 13C-labeled acetate (13C Acetate, red arrows) has a molecular mass of 61. a M. acetivorans harboring empty plasmid pES1(Pmat) grown on 13C-labeled methane (13CH4) for 10 days. b M. acetivorans harboring pES1-MATmcr3 grown on 13CH4 for 10 days. Note that the y-axes differ for a and b. c NMR HSQC spectrum identifying acetate by showing the one bond correlation between the methyl protons and the methyl carbon of acetate (13 CH 3 -C). d NMR HMBC spectrum identifying acetate in an independent manner by showing the two bonds correlation between the methyl protons and the carboxylic carbon of acetate (13CH 3 -COO). The x-axes denote 1H NMR plots, whereas the y-axes denote 13C NMR plots

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