Fig. 3
From: Reversing methanogenesis to capture methane for liquid biofuel precursors
Biofilm growth of ANME-1 Mcr-producing M. acetivorans on methane. a Representative hemocytometer views (×400 magnification) of M. acetivorans/pES1-MATmcr3 cells growing as biofilms on the precipitates after incubation on methane and 10 mM FeCl3. All cultures were stained with SYTO9 dye for microscopic examination. Under epifluorescence settings, cells appear green but abiotic precipitates do not fluoresce. Scale bars 20 µm. b Electron micrograph of irregular cocci M. acetivorans/pES1-MATmcr1 growing on methane and 0.1 mM FeCl3. Scale bar 0.2 µm. c Electron micrograph of irregular-cocci M. acetivorans grown on methanol. Scale bar 0.5 µm. Red arrows show the mono-layered cell wall