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Table 1 Plasmids and strains used in this study

From: Direct conversion of theophylline to 3-methylxanthine by metabolically engineered E. coli

Name Characteristics Source
Plasmids
 pAD1 AmpR, T7 promoter, ndmA, ndmD, rbsAD1, F1 origin [44]
 pET28-His-ndmD KanR, T7 promoter, ndmD, F1 origin [43]
 pACYCDuet-1 Expression vector, CmR, 2 T7 promoters, p15A origin Novagen
 dA pACYCDuet-1 with one copy of ndmA This study
 dA0 pACYCDuet-1 with one copy of ndmA and a second MCS This study
 dAA pACYCDuet-1 with two copies of ndmA This study
 dD0 pACYCDuet-1 with one copy of ndmD and a second MCS This study
 dDD pACYCDuet-1 with two copies of ndmD This study
 dDA pACYCDuet-1 with one copy of ndmD and on copy of ndmA This study
E. coli strains
 E. coli BL21(DE3) F ompT hsdS B (r B m B ) gal dcm (DE3) Invitrogen
 E. coli pAD1a BL21(DE3) pAD1 [44]
 E. coli pAD1dDD BL21(DE3) pAD1 dDD This study
 E. coli pAD1dDA BL21(DE3) pAD1 dDA This study
 E. coli pAD1dAA BL21(DE3) pAD1 dAA This study
 E. coli dDA BL21(DE3) dDA This study
 E. coli pHisD BL21(DE3) pET28-His-ndmD [43]
 E. coli pDdAA BL21(DE3) pET28-His-ndmD dAA This study
 E. coli pDdA BL21(DE3) pET28-His-ndmD dA This study
  1. aStrain pAD1 was originally named E. coli strain RMS1 in a previous publication [44]