Table 1 Plasmids and strains used in this study
From: Direct conversion of theophylline to 3-methylxanthine by metabolically engineered E. coli
Name | Characteristics | Source |
|---|---|---|
Plasmids | ||
 pAD1 | AmpR, T7 promoter, ndmA, ndmD, rbsAD1, F1 origin | [44] |
 pET28-His-ndmD | KanR, T7 promoter, ndmD, F1 origin | [43] |
 pACYCDuet-1 | Expression vector, CmR, 2 T7 promoters, p15A origin | Novagen |
 dA | pACYCDuet-1 with one copy of ndmA | This study |
 dA0 | pACYCDuet-1 with one copy of ndmA and a second MCS | This study |
 dAA | pACYCDuet-1 with two copies of ndmA | This study |
 dD0 | pACYCDuet-1 with one copy of ndmD and a second MCS | This study |
 dDD | pACYCDuet-1 with two copies of ndmD | This study |
 dDA | pACYCDuet-1 with one copy of ndmD and on copy of ndmA | This study |
E. coli strains | ||
 E. coli BL21(DE3) | F− ompT hsdS B (r −B m −B ) gal dcm (DE3) | Invitrogen |
 E. coli pAD1a | BL21(DE3) pAD1 | [44] |
 E. coli pAD1dDD | BL21(DE3) pAD1 dDD | This study |
 E. coli pAD1dDA | BL21(DE3) pAD1 dDA | This study |
 E. coli pAD1dAA | BL21(DE3) pAD1 dAA | This study |
 E. coli dDA | BL21(DE3) dDA | This study |
 E. coli pHisD | BL21(DE3) pET28-His-ndmD | [43] |
 E. coli pDdAA | BL21(DE3) pET28-His-ndmD dAA | This study |
 E. coli pDdA | BL21(DE3) pET28-His-ndmD dA | This study |