Fig. 2
From: Direct conversion of theophylline to 3-methylxanthine by metabolically engineered E. coli
Degradation of TP by metabolically engineered E. coli resting cells. Shaded triangle strain BL21(DE3) (negative control); Open circle strain pAD1; Shaded circle strain pAD1dAA; Open triangle strain dDA; Shaded square strain pAD1dDA; Open square strain pAD1dDD. Cells (OD600 = 2.5) were incubated with 1 mM TP in 50 mM KPi buffer at 30 °C with 400 rpm shaking, and metabolites were quantified via HPLC