Fig. 1
Construction of recombinant plasmid pSIP409-ACEIP. a Designed peptide sequences according to the synthesized oligonucleotides. Black bold, peptides of tuna frame protein; Black bold italic, arginine linker; Underlined, peptides of limanda aspera frame protein; Black bold underlined, His tag. b Illustration of pSIP409-ACEIP. Encoding sequences of angiotensin-converting enzyme inhibitory peptide (ACEIP) were digested by NcoI and HindIII and were ligated with pSIP409 that was digested with the same enzymes. 256rep replication origin for Lactobacillus, ermL erythromycin-resistance marker, P sppIP and P orfX inducible promoters, sppK and sppR histidine protein kinase and response regulator, respectively