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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Metabolic engineering of Mortierella alpina for arachidonic acid production with glycerol as carbon source

Fig. 4

Double-overexpression of GK and co-overexpression of GK and G3PD3 in M. alpina. The expression level (a), enzymatic activity (b) and total fatty acid level (c) in M. alpina strains were analyzed after double-overexpression of GK and co-overexpression of GK and G3PD3. M. alpina (black bars): wild type M. alpina; MA-gk-gk-1, MA-gk-gk-2, MA-gk-gk-3 (gray bars): GK double-overexpressing M. alpina strain; MA-g3pd3-Gk-1, MA-g3pd3-Gk-2, MA-g3pd3-Gk-3 (white bars): GK and G3PD3 co-overexpressing M. alpina strain; M. alpina (G): M. alpina growing with glucose as carbon source. Strains were cultured in 500 mL shaking flasks containing 200 mL Kendrick medium supplemented with 50 g/L glycerol for 168 h at 200 rpm. Three independent experiments were performed, and the error bars represent standard deviations. *p < 0.05 compared to wild type

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