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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Metabolic engineering of Mortierella alpina for arachidonic acid production with glycerol as carbon source

Fig. 3

Overexpression of GK and G3PDs in M. alpina. The expression level (a), enzymatic activity (b) and total fatty acid level (c) in M. alpina strains were analyzed after overexpressing GK and G3PDs. M. alpina (black bars): wild type M. alpina; MA-gk-1, MA-gk-2, MA-gk-3 (gray bars): GK-overexpressing M. alpina strain; MA-g3pd1-1, MA- g3pd1-2, MA-g3pd1-3 (cross-hatched bars): G3PD1-overexpressing M. alpina strain; MA-g3pd2-1, MA-g3pd2-2, MA-g3pd2-3 (striped bars): G3PD2-overexpressing M. alpina strain; MA-g3pd3-1, MA-g3pd3-2, MA-g3pd3-3 (white bars): G3PD3-overexpressing M. alpina strain; M. alpina (G): M. alpina growing with glucose as carbon source. Strains were cultured in 500 mL shaking flasks containing 200 mL Kendrick medium supplemented with 50 g/L glycerol for 168 h at 200 rpm. Three independent experiments were performed, and the error bars represent standard deviations. *p < 0.05 compared to wild type

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