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Table 2 Bacterial strains and plasmids used in this work

From: The secreted l-arabinose isomerase displays anti-hyperglycemic effects in mice

Strains

Genotypes

References

MRS36

Escherichia coli BL21 strain harboring the pMR36 plasmid

[30]

NZ9000

MG1363 (nisRK genes in chromosome), plasmid free

[33]

NZ9000 htrA

NZ9000 defective for the htrA gene, plasmid free

[29]

NZ9000 clpP

NZ9000 defective for the clpP gene, plasmid free

[34]

NZ9000 htrA clpP

NZ9000 defective for the htrA and the clpP genes, plasmid free

[35]

MRS37

NZ9000 harbouring the psec:LEISS:araA

This work

MRS38

NZ9000 clpP harbouring the psec:LEISS:araA

This work

MRS39

NZ9000 htrA harbouring the psec:LEISS:araA

This work

MRS40

NZ9000 htrAclpP harbouring the psec:LEISS:araA

This work

Plasmid

Construct

References

pMR36

Plasmid encoding the l-arabinose isomerase encoding gene (araA) from L. sakei 23 K

[30]

pCYT

Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter

[22]

pSEC

Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter fused to the signal peptide of the usp45 gene

[22]

pSEC:LEISS

Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter in front of the signal peptide of usp45 fused to a sequence encoding the LEISTCDA polypeptide

[22]

pCYT:araA

pCyt carrying the L. sakei araA gene under the control of the pnis promoter

This work

pSEC:araA

pSec carrying the L. sakei araA gene fused to the signal peptide of usp45and under the control of the pnis promoter

This work

pSEC:LEISS:araA

pSec:LEISS carrying under the control of the pnis promoter, the signal peptide of usp45 associated with the LEISSTCDA encoding fragment and fused to the L. sakei araA gene

This work