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Table 2 Bacterial strains and plasmids used in this work

From: The secreted l-arabinose isomerase displays anti-hyperglycemic effects in mice

Strains Genotypes References
MRS36 Escherichia coli BL21 strain harboring the pMR36 plasmid [30]
NZ9000 MG1363 (nisRK genes in chromosome), plasmid free [33]
NZ9000 htrA NZ9000 defective for the htrA gene, plasmid free [29]
NZ9000 clpP NZ9000 defective for the clpP gene, plasmid free [34]
NZ9000 htrA clpP NZ9000 defective for the htrA and the clpP genes, plasmid free [35]
MRS37 NZ9000 harbouring the psec:LEISS:araA This work
MRS38 NZ9000 clpP harbouring the psec:LEISS:araA This work
MRS39 NZ9000 htrA harbouring the psec:LEISS:araA This work
MRS40 NZ9000 htrAclpP harbouring the psec:LEISS:araA This work
Plasmid Construct References
pMR36 Plasmid encoding the l-arabinose isomerase encoding gene (araA) from L. sakei 23 K [30]
pCYT Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter [22]
pSEC Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter fused to the signal peptide of the usp45 gene [22]
pSEC:LEISS Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter in front of the signal peptide of usp45 fused to a sequence encoding the LEISTCDA polypeptide [22]
pCYT:araA pCyt carrying the L. sakei araA gene under the control of the pnis promoter This work
pSEC:araA pSec carrying the L. sakei araA gene fused to the signal peptide of usp45and under the control of the pnis promoter This work
pSEC:LEISS:araA pSec:LEISS carrying under the control of the pnis promoter, the signal peptide of usp45 associated with the LEISSTCDA encoding fragment and fused to the L. sakei araA gene This work