From: The secreted l-arabinose isomerase displays anti-hyperglycemic effects in mice
Strains | Genotypes | References |
---|---|---|
MRS36 | Escherichia coli BL21 strain harboring the pMR36 plasmid | [30] |
NZ9000 | MG1363 (nisRK genes in chromosome), plasmid free | [33] |
NZ9000 htrA | NZ9000 defective for the htrA gene, plasmid free | [29] |
NZ9000 clpP | NZ9000 defective for the clpP gene, plasmid free | [34] |
NZ9000 htrA clpP | NZ9000 defective for the htrA and the clpP genes, plasmid free | [35] |
MRS37 | NZ9000 harbouring the psec:LEISS:araA | This work |
MRS38 | NZ9000 clpP harbouring the psec:LEISS:araA | This work |
MRS39 | NZ9000 htrA harbouring the psec:LEISS:araA | This work |
MRS40 | NZ9000 htrAclpP harbouring the psec:LEISS:araA | This work |
Plasmid | Construct | References |
---|---|---|
pMR36 | Plasmid encoding the l-arabinose isomerase encoding gene (araA) from L. sakei 23 K | [30] |
pCYT | Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter | [22] |
pSEC | Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter fused to the signal peptide of the usp45 gene | [22] |
pSEC:LEISS | Vector derived from pGK12 carrying a chloramphenicol resistance gene and the pnis inducible promoter in front of the signal peptide of usp45 fused to a sequence encoding the LEISTCDA polypeptide | [22] |
pCYT:araA | pCyt carrying the L. sakei araA gene under the control of the pnis promoter | This work |
pSEC:araA | pSec carrying the L. sakei araA gene fused to the signal peptide of usp45and under the control of the pnis promoter | This work |
pSEC:LEISS:araA | pSec:LEISS carrying under the control of the pnis promoter, the signal peptide of usp45 associated with the LEISSTCDA encoding fragment and fused to the L. sakei araA gene | This work |