Table 1 Biotransformation of 1,3PDO by G. oxydans at 28 °C and 800 rpm for 3 h
Biocatalyst (mg/mL) | Alcohol dehydrogenase reaction (ADH) | Aldehyde dehydrogenase reaction (ALDH) | 3HP Yield d mol% (24Â h) | |||||
|---|---|---|---|---|---|---|---|---|
1,3PDO (mg/mL) | Remaining 1,3PDO (mg/mL) | Conversiona (%) | Substrateb (mg/mL) | 3HPA (mg/mL) | 3HP (mg/mL) | Conversionc (%) | ||
5.2 | 5 | 0.2 | 95.3 | 4.6 | 0.2 | 5.4 | 95.0 | 95.4 |
5.2 | 10 | 1.4 | 86.1 | 8.4 | 0.1 | 10.1 | 98.9 | 96.9 |
5.2 | 15 | 7.7 | 48.7 | 7.1 | 0.1 | 8.6 | 99.1 | 97.1 |
5.2 | 20 | 13.7 | 31.3 | 6.1 | 0.1 | 7.3 | 98.6 | 96.6 |
5.2 | 25 | 18.9 | 24.5 | 6.0 | 1.4 | 5.5 | 76.0 | 98.5 |
5.2 | 30 | 24.8 | 17.2 | 5.0 | 1.6 | 4.2 | 68.0 | 98.3 |
2.6 | 10 | 4.5 | 54.9 | 5.4 | 0.0 | 6.5 | 99.7 | 98.5e |
3.9 | 10 | 2.4 | 75.7 | 7.4 | 0.1 | 8.9 | 99.0 | 97.8e |
6.5 | 10 | 2.0 | 80.2 | 7.8 | 0.2 | 9.3 | 98.1 | 95.8e |