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Table 1 Yield and binding capacity of the proof of concept PhaC-SrtA-Target PHA beads

From: In vivo polyester immobilized sortase for tagless protein purification

Construct mg target protein eluted per g wet bead MW of target (g/mole) nmoles target per g wet bead g Wet beads/L mg target protein/L nmoles protein/L g dry cells/L % PHA by GCMS
PhaC-Srt-LPETG-GFP 2.84 26,820 106.03 2.14 6.08 226.9 3.870 27.50
PhaC-Srt-LPETG-MBP 2.23 40,254 55.29 2.24 5.00 123.85 3.740 20.66
PhaC 0.00 2.68 3.488 30.72