Table 1 Yield and binding capacity of the proof of concept PhaC-SrtA-Target PHA beads
From: In vivo polyester immobilized sortase for tagless protein purification
Construct | mg target protein eluted per g wet bead | MW of target (g/mole) | nmoles target per g wet bead | g Wet beads/L | mg target protein/L | nmoles protein/L | g dry cells/L | % PHA by GCMS |
|---|---|---|---|---|---|---|---|---|
PhaC-Srt-LPETG-GFP | 2.84 | 26,820 | 106.03 | 2.14 | 6.08 | 226.9 | 3.870 | 27.50 |
PhaC-Srt-LPETG-MBP | 2.23 | 40,254 | 55.29 | 2.24 | 5.00 | 123.85 | 3.740 | 20.66 |
PhaC | 0.00 | – | – | 2.68 | – | – | 3.488 | 30.72 |