Fig. 4
From: In vivo polyester immobilized sortase for tagless protein purification
Purification of RV1626 from the PhaC-SrtA-Target beads. SDS-PAGE of the PHA bead and soluble fraction before and after activation with CaCl2 and triglycine at different time points. PST PhaC-SrtA-Target band (pre cleavage); PS PhaC-SrtA band (post cleavage); T target protein