Fig. 3
From: In vivo polyester immobilized sortase for tagless protein purification
Purification of GFP (A), MBP (B), and RV1622 from the PhaC-SrtA-Target beads. SDS-PAGE of the PHA bead and soluble fraction before and after activation with CaCl2 and triglycine at different time points. PST PhaC-SrtA-Target band (pre cleavage); PS PhaC-SrtA band (post cleavage); T target protein