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Table 3 RT-qPCR mRNA expression values of PtdEtn degradation, β-oxidation and phospholipid biosynthesis genes in the evolved strains

From: Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds

Genes

PB11

PB12

PB13

2-Acyl-glycerophosphoethanolamine cycle

 aas

1.03 ± 0.05

0

0

 lnt

1.09 ± 0.26

1.47 ± 0.03

2.43 ± 0.46

 lplT

0.97 ± 0.08

0

0

 msbA

1.44 ± 0.08

2.25 ± 0.24

3.87 ± 0.71

PtdEtn degradation

 fadD

1.2 ± 0.26

0.66 ± 0.1

0.95 ± 0.26

 glpQ

1.44 ± 0.39

1.89 ± 0.35

1.90 ± 0.10

 glpT

2.59 ± 0.40

2.22 ± 0.05

63.46 ± 7.17

 lnt

1.09 ± 0.26

1.47 ± 0.03

2.43 ± 0.46

 pldA

1.71 ± 0.30

2.85 ± 0.08

4.79 ± 1.10

 pldB

0.74 ± 0.05

1.57 ± 0.28

1.65 ± 0.13

β-oxidation

 fadA

30.50 ± 0.21

6.83 ± 1.07

7.52 ± 0.71

 fadB

33.13 ± 2.89

6.20 ± 1.03

4.12 ± 0.69

 fadD

1.2 ± 0.26

0.66 ± 0.1

0.95 ± 0.26

 fadE

9.12 ± 1.43

56.93 ± 1.75

13.68 ± 1.56

 fadL

2.14 ± 0.07

1.34 ± 0.02

2.12 ± 0.53

 fadR

2.66 ± 0.40

3.00 ± 0.16

4.48 ± 0.22

Fatty acid biosynthesis

 accA

1.08 ± 0.20

0.64 ± 0.04

0.62 ± 0.09

 accB

0.77 ± 0.05

0.78 ± 0.05

0.67 ± 0.10

 accD

0.64 ± 0.02

0.42 ± 0.04

0.23 ± 0.05

 fabA

2.25 ± 0.18

3.03 ± 0.16

4.21 ± 0.11

 fabB

1.99 ± 0.22

1.26 ± 0.12

2.63 ± 0.33

 fabD

1.25 ± 0.03

1.50 ± 0.05

1.95 ± 0.11

 fabF

0.66 ± 0.06

1.54 ± 0.05

2.88 ± 0.04

 fabG

0.69 ± 0.01

1.38 ± 0.11

1.74 ± 0.07

 fabH

1.02 ± 0.19

1.30 ± 0.24

2.05 ± 0.21

 fabI

2.04 ± 0.13

5.01 ± 0.20

3.00 ± 0.00

 fabR

0.90 ± 0.06

1.72 ± 0.11

2.12 ± 0.28

 fabZ

0.59 ± 0.07

1.84 ± 0.01

1.91 ± 0.24

Phospholipid biosynthesis

 plsB

2.30 ± 0.13

2.12 ± 0.50

2.52 ± 0.31

 plsC

0.52 ± 0.06

1.67 ± 0.12

2.35 ± 0.18

 plsX

2.39 ± 0.15

2.34 ± 0.28

2.55 ± 0.08

  1. Relative mRNA concentrations of PB11, PB12 and PB13 strains, grown on glucose as the sole carbon source were determined by RT-qPCR. Data in this table are reported as relative expression levels of the parental strain JM101. The mRNA level of each gene in the parental strain was used as control to normalize the data, assigning it the value of one. Central carbon metabolism genes and other important genes have been previously reported and are listed in Additional file 4: Table S3
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Microbial Cell Factories

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