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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds

Fig. 1

Adaptive laboratory experiment. a Isolation of evolved strains from a continuous culture of the PB11 from Aguilar et al. [2]. The arrows indicate the isolation time for various strains including PB12 and PB13 strains. Dotted line indicates the end of the batch culture and the start of the continuous culture; numbers indicate dilution rates (D = h−1) as follows: 1 for D = 0.4, 2 for D = 0.6 and 3 for D = 0.8. b Chromosomal gene organization in the parental wild type JM101 strain and in the laboratory evolved PB12 and PB13 strains. c PCR test for the chromosomal deletion in the evolved strains isolated at D = 0.4, 0.6 and 0.8, where the absence of the 10,328 bp chromosomal DNA fragment can be observed in 6 strains. Line 1, (M) molecular weight marker; lines 2 and 3, amplification of the chromosomal region in the JM101 and PB11 strains controls respectively (12 kb); lines 49, amplification of the 1.9 kb from the chromosomal region in the six strains isolated during the continuous culture as follows: line 4 and 5, PB12 and the second strain isolated at D = 0.4, line 6 and 7, first and second strains isolated at D = 0.6, line 8 and 9, first strain isolated at D = 0.8 and PB13 strain; line 10, (M) molecular weight marker

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