Fig. 3

rpoS knockout causes oxidative stress in stationary phase. Cells were grown in the presence of the H₂O₂-activated fluorescent dye DHR123 and subjected to flow cytometry to measure intracellular ROS. Peaks represent distribution of fluorescence intensity from ten thousand cells per replicate (n = 3 replicates). The panels show DHR123-dependent fluorescence in MG1655* and Seq⁺ during exponential, early and late stationary growth phase, respectively