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Table 3 Kinetic parameters of PhaCNSDG in insoluble fractions of R. eturopha recombinant strains toward (R)-3HB-CoA (C4) and (R)-3HHx-CoA (C6)

From: Compositional regulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by replacement of granule-associated protein in Ralstonia eutropha

Strain (genotype)

Substrate chain length

Km

Vmax

Vmax/Km

(µM)

C6/C4 ratio

(µmol/min/mg-insolublea)

C6/C4 ratio

 

C6/C4 ratio

NSDG-P1ReCJ

C4

320

1.9

0.21

0.20

6.4 × 10−4

0.10

(phaP1 Re -phaC NSDG-phaJ Ac )

C6

614

0.041

6.6 × 10−5

NSDG-PAcCJ

C4

86

0.71

0.52

0.21

6.1 × 10−3

0.30

(phaP Ac -phaC NSDG-phaJ Ac )

C6

61

0.11

1.8 × 10−3

  1. The insoluble fractions were prepared from the cells cultivated in an MB medium containing 1 % (v/v) soybean oil at 30 °C for 48 h
  2. aDry weight of the insoluble fraction
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Microbial Cell Factories

ISSN: 1475-2859

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