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Table 2 Computation predictions for different modes of methane oxidation and growth conditions

From: Genome-scale metabolic reconstructions and theoretical investigation of methane conversion in Methylomicrobium buryatense strain 5G(B1)

  Biomass fluxa Oxygen consumption O2:CH4 ratio CO2 production CCE
Unconstrained network 0.246 21.34 1.16 7.78 57.85
Methane oxidation mode
 Direct coupling 0.242 21.59 1.17 7.95 56.93
 Direct coupling/low ATP maintenance 0.263 20.24 1.10 7.02 61.97
 Direct coupling/low ATP yield 0.238 21.82 1.18 8.1 56.12
 Low efficiency direct coupling, complemented by NADH (1 mol of NADH per 10 mol of methane oxidized)b 0.23 21.43 1.16 8.47 54.12
 Low efficiency direct coupling, complemented by NADH (1 mol of NADH per 5 mol of methane oxidized) 0.219 21.2 1.15 8.94 51.57
 Uphill electron transfer/ NADH input (2/3 electrons from cytochrome cL and 1/3 from NADH) 0.224 21.8 1.18 8.72 52.76
 Uphill electron transfer/NADH input (1/2 electrons from cytochrome cL and 1/2 from NADH) 0.205 22.99 1.25 9.53 48.37
 Redox arm (0.5 mol ATP per 1 mol methanol oxidized) 0.146 27.69 1.50 12.13 34.29
 Redox arm (1 mol ATP per 1 mol of methanol oxidized) 0.146 27.69 1.50 12.13 34.29
 Redox arm (1 mol ATP per 1 mol of methanol oxidized)/low ATP maintenance 0.146 27.69 1.50 12.13 34.29
 Redox arm (0.5 mol ATP per 1 mol methanol oxidized)/high ATP maintenance 0.142 27.92 1.51 12.29 33.42
Assimilation pathways (with low efficiency direct coupling complemented by NADH)a
 EMP only 0.232 21.28 1.15 8.37 54.66
 EDD only 0.223 21.85 1.18 8.76 52.55
 BS (acetyl-CoA synthesis only) 0.228 21.55 1.17 8.56 53.63
 Serine cycle (acetyl-CoA synthesis only) 0.222 21.93 1.19 8.82 52.22
Nitrogen source
 Nitrogen fixation 0.228 23.23 1.26 8.58 53.52
 Ammonium 0.29 22.08 1.20 5.88 68.15
 Ureac 0.293 21.93 1.19 6.86 62.84
Sulfur source
 HS 0.233 21.35 1.16 8.37 54.66
 HS and Ureac 0.295 21.93 1.19 6.79 63.22
  1. aMethane uptake is 18.46 mmol g CDW −1 h−1
  2. bMethane oxidation mode used to calculate data shown here for different sources of nitrogen and sulfur
  3. cOnly CO2 from methane was used to calculate CCE