Table 1 The biomass composition and general growth parameters of the M. buryatense 5G(B1) cells

Amino acids

54.8

3.1

M. buryatense 5GB1

 Alanine

0.446

This study

 Arginine

0.202

This study

 Asparagine

0.119

This study

 Aspartate

0.348

This study

 Cysteine

0.03

This study

 Glutamate

0.396

This study

 Glutamine

0.15

This study

 Histidine

0.088

This study

 Glycine

0.443

This study

 Isoleucine

0.256

This study

 Leucine

0.26

This study

 Lysine

0.25

This study

 Methionine

0.12

This study

 Phenylalanine

0.196

This study

 Proline

0.195

This study

 Serine

0.23

This study

 Threonine

0.26

This study

 Tryptophan

0.072

This study

 Tyrosine

0.14

This study

 Valine

0.341

This study

Ectoine

0.38

0.1

0.027

M. buryatense 5G

[23, 24]

Lipids

 FAME

10.9

0.56

M. buryatense 5GB1

This study

  C14 (myristate)

0.016

This study

  C15 (pentadecylic acid)

0.002

This study

  C16 (palmitate)

0.307

This study

  C18 (stearate)

0.001

This study

 Phospholipids

M. buryatense 5G

Recalculated from [23]

  Phosphatidylserine*

0.014

[23]

  Phosphatidylethanolamine*

0.122

[23]

  Dipalmitoyl phosphatidate*

0.007

[23]

  Phosphatidylglycerol*

0.031

[23]

 Sterols

0.08

–

0.08

M. buryatense 5GB1

This study

  squalene

0.04

  lanosterol

0.04

Intracellular metabolites

1.5

–

Ribulose-5-phosphate/ribose-5-phosphate

0.001

M. alcaliphilum 20Z

[42]

Fructose-1, 6-bisphosphate

0.001

M. alcaliphilum 20Z

[42]

Fructose-6-phosphate

0.003

M. alcaliphilum 20Z

[42]

Glucose-6-phosphate

0.002

M. alcaliphilum 20Z

[42]

Glyceraldehyde-3-phosphate/dihydroxyacetone

0.003

M. alcaliphilum 20Z

[42]

6-Phosphogluconic acid

0.00015

M. alcaliphilum 20Z

[42]

2-Dehydro-3-deoxy-phosphogluconate

0.000003

M. alcaliphilum 20Z

[42]

Phosphoglycerate

0.006

M. alcaliphilum 20Z

[42]

Phosphoenolpyruvate

0.005

M. alcaliphilum 20Z

[42]

Pyruvate

0.015

M. alcaliphilum 20Z

[42]

Acetyl-CoA

0.0001

M. alcaliphilum 20Z

[42]

Succinate

0.002

M. alcaliphilum 20Z

[42]

Malate

0.004

M. alcaliphilum 20Z

[42]

Fumarate

0.001

M. alcaliphilum 20Z

[42]

Citrate

0.001

M. alcaliphilum 20Z

[42]

Glycerate

0.001

M. alcaliphilum 20Z

[42]

 ATP

0.005

Methylomonas methanica

[43, 44]

 ADP

0.002

M. extorquens AM1

[16]

 AMP

0.001

M. extorquens AM1

[16]

 NAD

0.002

M. extorquens AM1

[16]

 NADH

0.002

M. extorquens AM1

[16]

 NADP

0.001

M. extorquens AM1

[16]

 NADPH

0.001

M. extorquens AM1

[16]

 polyP (PPi)

0.029

Methylomonas methanica

[43, 44]

Cofactors

 Cytochrome c

0.00036

M. alcaliphilum 20Z

[37]

 B12

0.00000006

M. alcaliphilum 20Z

[41]

 Ubiquinol-8

0.00022

Assumption

[16]

 Protoheme

0.00022

Assumption

[16]

 coenzyme-A

0.00022

Assumption

[16]

 FMN

0.00022

Assumption

[16]

 FMNH2

0.00022

Assumption

[16]

 FAD

0.00022

Assumption

[16]

 SAM

0.00022

Assumption

[16]

 Glutathione

0.00022

Assumption

[16]

Carbohydrates

3.78

1.56

 Mannose

0.002

M. alcaliphilum 20Z

[24]

 Ramnose

0.0002

M. alcaliphilum 20Z

[24]

 Glycogen

0.117

M. buryatense 5GB1

[26]

 Sucrose

0.01

M. alcaliphilum 20Z

[45]

 Ribose

0.036

M. alcaliphilum 20Z

[24]

 Maltose

0.008

M. alcaliphilum 20Z

[24]

 Arabinose

0.022

M. alcaliphilum 20Z

[24]

 Galactose

0.005

M. alcaliphilum 20Z

[24]

Cell wall

9.127

 Peptidoglycan

–

0.053

Escherichia coli

[46]

 LPS (lipid IVA and KDO)

–

0.002

M. buryatense 5GB1

This study

RNA

9.7

3.6

M. buryatense 5GB1

This study

 ATP

0.050

 UTP

0.050

 CTP

0.047

 GTP

0.047

DNA

4.0

0.28

M. buryatense 5GB1

This study

 dATP

0.0021

 dTTP

0.0021

 dCTP

0.0020

 dGTP

0.0020

Ash (without P and S data)

5.2

1.03

–

M. buryatense 5GB1

[27]

 Copper

0.01

 Magnesium

0.07

 Iron

0.0059

 Cobalt

0.001

 Calcium

0.01

Biomass (measured)

99.5

10.2

3-PG

0.5

0.003

Total

100.0

Excreted products

 Formate

2.8

0.17

0.6

M. buryatense 5GB1

[27]

 Acetate

0.7

0.02

0.114

M. buryatense 5GB1

[27]

 Lactate

0.4

0.13

0.044

M. buryatense 5GB1

[27]

 EPS

10

4.5

M. buryatense 5GB1

This study

  Glucose

0.0420

M. buryatense 5GB1

This study

  Fucose

0.0075

M. buryatense 5GB1

This study

  Xylose

0.0023

M. buryatense 5GB1

This study

  Inositol

0.0021

M. buryatense 5GB1

This study

  Galactose

0.0588

M. buryatense 5GB1

This study

  Mannose

0.0669

M. buryatense 5GB1

This study

  Ribose

0.0342

M. buryatense 5GB1

This study

  Rhamnose

0.0432

M. buryatense 5GB1

This study

  Glucosamine

0.0415

M. buryatense 5GB1

This study

  Galactosamine

0.0394

M. buryatense 5GB1

This study

  Cysteine

0.0185

M. buryatense 5GB1

This study

  Threonine

0.0375

M. buryatense 5GB1

This study

  Serine

0.0214

M. buryatense 5GB1

This study

  Glutamate

0.0319

M. buryatense 5GB1

This study

  Glycine

0.0830

M. buryatense 5GB1

This study

  Valine

0.0228

M. buryatense 5GB1

This study

  Methionine

0.0066

M. buryatense 5GB1

This study

  Isoleucine

0.0146

M. buryatense 5GB1

This study

  Leucine

0.0169

M. buryatense 5GB1

This study

  Phenylalanine

0.0139

M. buryatense 5GB1

This study

Growth parameters

 Methane uptake (mmol g CDW⁻¹ h⁻¹)

18.46 ± 1.36

M. buryatense 5GB1

[27]

 Oxygen uptake (mmol g CDW⁻¹ h⁻¹)

23.55 ± 1.13

M. buryatense 5GB1

[27]

 O₂/CH₄ uptake Ratio

1.25 ± 0.05

M. buryatense 5GB1

[27]

 Specific growth rate (h⁻¹)

0.232 ± 0.006

M. buryatense 5GB1

[27]