Table 1 Strains, plasmids, gene and primers
Bacteria, plasmid, or gene | Relevant features or sequence (5′–3′) | References or sources |
|---|---|---|
Bacteria | ||
E. coli DH5α | endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR F- Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK +), λ– | Invitrogen |
L. lactis NZ9000 | MG1363 nisRK ΔpepN | |
Lb. plantarum ATCC 8014 | Wild type | ATCC |
Plasmid | ||
pNZ8148 | pSH71 derivative, PnisA, CmR, nisin-controlled expression | |
pNZRK | pNZ8148 containing nisR and nisK genes | This work |
pNZ-IRFP713 | pNZ8148 containing irfp713 gene | This work |
pNZ-IRFP682 | pNZ8148 containing irfp682 gene | This work |
pNZRK-IRFP713 | pNZ8148 containing irfp713, nisR and nisK genes | This work |
pMA-T-IRFP713 | pMA-T containing irfp713 gene | This work |
pMSP3545 | Emr, P nisA , nisRK, NcoI for translational fusion, ColE1 and pAMβ1 replicons | [37] |
pMSP3545-IRFP713 | pMSP3545 containing irfp713 gene | This work |
pGEM::CP25-IRFP | pGEM-T Easy containing irfp713 gene under the control of CP25 promoter | [27] |
Gene | ||
irfp713 | CCATGGCTGAGGGATCTGTAGCTCGTCAACCTGATTTACTTACTTGTGACGATGAACCTATTCATATTCCAGGTGCTATTCAACCACACGGACTTTTATTAGCTCTTGCCGCTGACATGACTATCGTCGCTGGATCAGATAATTTACCTGAATTGACTGGTTTAGCAATTGGAGCCCTTATTGGACGATCAGCAGCAGATGTTTTTGATTCAGAAACTCATAATCGTCTTACTATTGCATTGGCAGAACCTGGTGCTGCAGTAGGTGCTCCTATTACAGTAGGTTTCACTATGCGTAAGGATGCTGGTTTTATTGGTTCTTGGCATCGTCATGATCAACTTATTTTTTTAGAGTTGGAACCACCACAAAGAGACGTTGCAGAGCCTCAAGCTTTTTTTCGTCGTACTAATTCAGCAATTCGTAGACTTCAAGCTGCTGAAACTCTTGAATCTGCATGTGCAGCAGCAGCACAAGAGGTACGAAAAATTACAGGTTTTGATAGAGTTATGATTTACAGATTTGCCTCAGACTTTTCTGGTGAAGTAATCGCAGAGGATAGATGTGCCGAGGTTGAAAGTAAATTGGGATTGCATTATCCTGCCAGTACTGTTCCAGCCCAAGCACGTCGTCTTTATACTATTAATCCTGTTAGAATTATCCCAGATATTAATTATCGACCAGTTCCAGTTACTCCTGACTTAAATCCTGTAACTGGTAGACCTATTGACTTGTCATTTGCCATCTTACGATCTGTTTCACCTGTTCATTTAGAGTTTATGCGTAATATTGGTATGCATGGTACTATGTCTATCTCAATCCTTCGAGGAGAACGTTTATGGGGACTTATTGTTTGTCATCATAGAACACCTTATTATGTCGATTTAGATGGACGTCAAGCTTGTGAATTAGTTGCTCAAGTCTTGGCTTGGCAAATTGGTGTAATGGAAGAATAATCTAGA | This work |
Primers | ||
nisR-F | GATGATAAGCTGTCCAAAC | This work |
nisK-R | TTTAGGATAACTTCTGCCC | This work |
682-1F | CTCAGACTTTTCTGGTGTAGTAATCGCAGAGGATAG | This work |
682-2F | GCATTATCCTGCCAGTGCTGTTCCAGCCCAAGC | This work |
682-3F | CCATCTTACGATCTGTTTCACCTTGTCATTTAGAGTTTATGCG | This work |
682-1R | CTATCCTCTGCGATTACTACACCAGAAAAGTCTGAG | This work |
682-2R | GCTTGGGCTGGAACAGCACTGGCAGGATAATGC | This work |
682-3R | CGCATAAACTCTAAATGACAAGGTGAAACAGATCGTAAGATGG | This work |