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Table 2 Parameters for continuous growth conditions in 1L bioreactor with substrate limitation

From: Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1

Experiment ID FM50 FM51 FM38 FM39
Limiting condition Oxygen limited Oxygen limited Methane limited Methane limited
Total FAME (% cell dry weight) 10.5 10.7 10.2 10.5
Dilution rate (h−1) 0.119 0.122 0.122 0.126
Cell density (gCDW L−1) 0.79 0.77 0.45 0.46
Dissolved oxygen (% of saturation) 1 1 92 92
Oxygen uptake (mmol h−1) 8.8 9.5 6.2 5.9
Methane uptake (mmol h−1) 7.7 8.2 3.8 3.6
Specific oxygen uptake (mmol gCDW−1 h−1) 11.1 12.3 13.7 12.8
Specific methane uptake (mmol gCDW−1 h−1) 9.7 10.6 8.4 7.8
O2/CH4 uptake ratio 1.1 1.2 1.6 1.6
Carbon conversion efficiency (%) 46 43 54 61
Excreted products
 Formate (μmol gCDW−1) 286 301 255 459
 Acetate (μmol gCDW−1) 36 85 32 28
 Lactate (μmol gCDW−1) 24 28 47 21
Glycogen content (% CDW) 7.2 ± 1.9 13.1 ± 4.0 6.0 ± 0.5 8.1 ± 2.5
Inlet gas composition 20 % CH4, 5 % O2, 75 % N2 20 % CH4, 5 % O2, 75 % N2 2.5 % CH4, 97.5 % Air 2.5 % CH4, 97.5 % Air
  1. Summary of process parameters and results for steady-state bioreactor culture under oxygen limitation and methane limitation. Completed in duplicate
  2. FAME fatty acid methyl ester