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Table 2 Parameters for continuous growth conditions in 1L bioreactor with substrate limitation

From: Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1

Experiment ID

FM50

FM51

FM38

FM39

Limiting condition

Oxygen limited

Oxygen limited

Methane limited

Methane limited

Total FAME (% cell dry weight)

10.5

10.7

10.2

10.5

Dilution rate (h−1)

0.119

0.122

0.122

0.126

Cell density (gCDW L−1)

0.79

0.77

0.45

0.46

Dissolved oxygen (% of saturation)

1

1

92

92

Oxygen uptake (mmol h−1)

8.8

9.5

6.2

5.9

Methane uptake (mmol h−1)

7.7

8.2

3.8

3.6

Specific oxygen uptake (mmol gCDW−1 h−1)

11.1

12.3

13.7

12.8

Specific methane uptake (mmol gCDW−1 h−1)

9.7

10.6

8.4

7.8

O2/CH4 uptake ratio

1.1

1.2

1.6

1.6

Carbon conversion efficiency (%)

46

43

54

61

Excreted products

 Formate (μmol gCDW−1)

286

301

255

459

 Acetate (μmol gCDW−1)

36

85

32

28

 Lactate (μmol gCDW−1)

24

28

47

21

Glycogen content (% CDW)

7.2 ± 1.9

13.1 ± 4.0

6.0 ± 0.5

8.1 ± 2.5

Inlet gas composition

20 % CH4, 5 % O2, 75 % N2

20 % CH4, 5 % O2, 75 % N2

2.5 % CH4, 97.5 % Air

2.5 % CH4, 97.5 % Air

  1. Summary of process parameters and results for steady-state bioreactor culture under oxygen limitation and methane limitation. Completed in duplicate
  2. FAME fatty acid methyl ester