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Table 1 Parameters for unrestricted growth in batch 1L bioreactor

From: Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1

Experiment ID FM03 FM21 FM18 FM23
Carbon source Methane Methane Methanol Methanol
Total FAME (% cell dry weight) 8.2 8.5 6.0 5.1
Specific growth rate (h−1) 0.239 0.224 0.169 0.173
Cell density (gCDW L−1) 0.34 0.31 0.81 0.54
Oxygen uptake (mmol h−1) 7.6 7.6 9.1 5.1
Methane uptake (mmol h−1) 5.8 6.1 N/A N/A
Specific oxygen uptake (mmol gCDW−1 h−1) 22.4 24.7 11.3 9.4
Specific methane uptake (mmol gCDW−1 h−1) 17.1 19.8 N/A N/A
O2/CH4 uptake ratio 1.3 1.2 N/A N/A
Excreted products
 Formate (μmol gCDW−1) 400 672 11,758 13,486
 Acetate (μmol gCDW−1) 96 123 55 56
 Lactate (μmol gCDW−1) 26 62 16 31
Glycogen content (% CDW) N/A 2.7 ± 1.1 N/A 42.8 ± 17.5
Inlet gas composition 10 % CH4, 5 % O2, 75 % N2 10 % CH4, 5 % O2, 75 %N2 0.5 % CH3OHa, Air 0.5 % CH3OHa, Air
  1. Summary of process parameters and results for two batch bioreactor experiments using methane and methanol as carbon source. Completed in duplicate
  2. Fed-batch gas uptake rates are at the time of harvest. Secreted products are measured at the time of harvest and represent accumulated concentration
  3. FAME Fatty Acid Methyl Ester
  4. aInitial methanol concentration by volume. No additional methanol is added during the experiment