Table 1 Parameters for unrestricted growth in batch 1L bioreactor

Total FAME (% cell dry weight)

8.2

8.5

6.0

5.1

Specific growth rate (h⁻¹)

0.239

0.224

0.169

0.173

Cell density (gCDW L⁻¹)

0.34

0.31

0.81

0.54

Oxygen uptake (mmol h⁻¹)

7.6

7.6

9.1

5.1

Methane uptake (mmol h⁻¹)

5.8

6.1

N/A

N/A

Specific oxygen uptake (mmol gCDW⁻¹ h⁻¹)

22.4

24.7

11.3

9.4

Specific methane uptake (mmol gCDW⁻¹ h⁻¹)

17.1

19.8

N/A

N/A

O₂/CH₄ uptake ratio

1.3

1.2

N/A

N/A

Excreted products

 Formate (μmol gCDW⁻¹)

400

672

11,758

13,486

 Acetate (μmol gCDW⁻¹)

96

123

55

56

 Lactate (μmol gCDW⁻¹)

26

62

16

31

Glycogen content (% CDW)

N/A

2.7 ± 1.1

N/A

42.8 ± 17.5

Inlet gas composition

10 % CH₄, 5 % O₂, 75 % N₂

10 % CH₄, 5 % O₂, 75 %N₂

0.5 % CH₃OH^a, Air

0.5 % CH₃OH^a, Air