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Table 2 Asymmetric synthesis of (R)-CHBE in aqueous/organic solvent (1:1) biphasic systems

From: Significantly improved solvent tolerance of Escherichia coli by global transcription machinery engineering

Strains

Enzyme activity (U/mg)

Yield (%)

ee (%)

GDH

CgCR

Aqueous/butyl acetate

Aqueous/toluene

E. coli/BmGDH-CgCR

9.5

0.9

79.5

85.5

>99

E. coli/BmGDH-CgCR/pHACMC9

8.9

0.8

100

94.6

>99

  1. Reaction conditions: 0.5 g wet cells, 250 g/L glucose, 100 g/L of ethyl 4-chloro-3-oxobutanoate (COBE) in 5 mL potassium phosphate buffer (0.1 mol/L, pH 6.5) and 5 mL organic solvent, in 30 °C shaker for 2 h
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Microbial Cell Factories

ISSN: 1475-2859

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