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Fig. 6 | Microbial Cell Factories

Fig. 6

From: BluB/CobT2 fusion enzyme activity reveals mechanisms responsible for production of active form of vitamin B12 by Propionibacterium freudenreichii

Fig. 6

BluB/CobT2 reaction product with adenine and NaMN was not detected. a The LC chromatograms of the 48 h reaction with BluB/CobT, adenine and NaMN (dotted) at pH 8.5 and of the BluB/CobT enzyme preparation (solid line). Chemical structures of isomers Ade-RP and AMP indistinguishable by LC–MS/MS are shown. See also Additional file 1: Figure S2. b Part of the ClustalW alignment of CobT enzymes from S. enterica [NCBI: AAL20920.1], L. reuteri [NCBI: ABQ83934.1], S. meliloti [NCBI: CAC46463.1], P. denitrificans [Swiss-Prot: P29935.3], P. acidipropionici [NCBI: AFV88734.1] and P. freudenreichii [NCBI: CBL56167.1]. The amino acids corresponding to S80 and Q88 of S. enterica CobT and critical for lower ligand substrate specificity [17] are marked in bold. See also Additional file 1: Table S1

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Microbial Cell Factories

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