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Table 3 Glucose (Glc) consumption rate (GCR), molar yield and productivity of 2,3-butanediol (2,3-BD), and carbon recovery (CR) by the control and engineered C. glutamicum strains

From: Engineering Corynebacterium glutamicum for the production of 2,3-butanediol

  GCR (nmol min−1 mg  CDW−1) Yield (mol 2,3-BD per mol Glc) Productivity (nmol min−1 mg CDW−1) CR (%)
ΔldhA (pEKEx2)* 2.5 ± 0.4 0.05 ± 0.00# 0.12 ± 0.01 81 ± 3
ΔldhA (pEKEx2- als,aldB,butA)* 4.4 ± 0.4 0.34 ± 0.04 1.28 ± 0.09 79 ± 9
ΔldhA (pEKEx2) 3.0 ± 0.2 0.02 ± 0.01# 0.06 ± 0.03 77 ± 1
ΔldhA (pEKEx2- als,aldB,butA) 3.4 ± 0.4 0.27 ± 0.06 0.53 ± 0.13 82 ± 3
ΔaceEΔpqoΔldhA (pEKEx2) 1.6 ± 0.3 0.22 ± 0.01# 0.30 ± 0.00 87 ± 1
ΔaceEΔpqoΔldhA (pEKEx2-als,aldB,butA) 5.4 ± 0.7 0.66 ± 0.05 3.55 ± 0.58 84 ± 7
ΔaceEΔpqoΔldhA (pEKEx2-als,aldB, Ptuf butA) 6.1 ± 0.1 0.61 ± 0.06 3.13 ± 0.22 87 ± 3
ΔaceEΔpqoΔldhAΔmdh (pEKEx2) 1.6 ± 0.3 0.34 ± 0.05# 0.53 ± 0.00 91 ± 6
ΔaceEΔpqoΔldhAΔmdh (pEKEx2-als,aldB,butA) 6.0 ± 0.3 0.64 ± 0.09 4.34 ± 0.16 90 ± 4
ΔaceEΔpqoΔldhAΔmdh (pEKEx2-als,aldB, Ptuf butA) 6.5 ± 0.5 0.64 ± 0.10 4.25 ± 0.41 91 ± 7
  1. In the first phase, cells were grown aerobically on 1 % (wt/vol) potassium acetate except for the strains indicated with an asterisk for which 0.5 % (wt/vol) glucose was used instead. Second phase fermentations were carried out for 48 h, using 25 mL of cell suspension in closed 50-mL falcon flasks with glucose as substrate. The cardinal symbol indicates that optically active 2,3-BD was produced; remaining strains produced meso-form of 2,3-BD. Values are averages of at least three independent experiments. A single NMR spectrum was acquired for each sample