Skip to main content

Advertisement

Table 2 Overexpression and specific activities of enzymes for the synthesis of 2,3-butanediol as determined in crude cell extracts

From: Engineering Corynebacterium glutamicum for the production of 2,3-butanediol

  ALS/ALDC activity U (mg of protein)−1 BDH activity U (mg of protein)−1 ALS/ALDC over-expression (-fold) BDH over-expression (-fold)
ΔldhA (pEKEx2)* 0.02 ± 0.00 0.66 ± 0.03
ΔldhA (pEKEx2-als,aldB,butA)* 1.47 ± 0.42 1.18 ± 0.26 61 2
ΔldhA (pEKEx2) 0.04 ± 0.00 0.20 ± 0.03
ΔldhA (pEKEx2-als,aldB,butA) 0.54 ± 0.09 0.10 ± 0.03 15
ΔaceEΔpqoΔldhA (pEKEx2) 0.03 ± 0.01 0.24 ± 0.04
ΔaceEΔpqoΔldhA (pEKEx2-als,aldB,butA) 0.58 ± 0.12 0.67 ± 0.01 17 3
ΔaceEΔpqoΔldhA (pEKEx2-als,aldB, Ptuf butA) 1.42 ± 0.24 0.53 ± 0.17 31 2
ΔaceEΔpqoΔldhAΔmdh (pEKEx2) 0.06 ± 0.02 0.11 ± 0.04
ΔaceEΔpqoΔldhAΔmdh (pEKEx2-als,aldB,butA) 0.43 ± 0.04 0.97 ± 0.19 8 8
ΔaceEΔpqoΔldhAΔmdh (pEKEx2-als,aldB, Ptuf butA) 0.47 ± 0.14 1.21 ± 0.01 9 10
  1. Cells were grown for 14 h in 2× TY medium supplemented with acetate, unless stated otherwise
  2. Values shown are averages ± SD of at least three independent experiments and two technical replicates
  3. ALS α-acetolactate synthase, ALDC α-acetolactate decarboxylase, BDH butanediol dehydrogenase
  4. * Cells grown on glucose