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Table 2 Overexpression and specific activities of enzymes for the synthesis of 2,3-butanediol as determined in crude cell extracts

From: Engineering Corynebacterium glutamicum for the production of 2,3-butanediol

 

ALS/ALDC activity U (mg of protein)−1

BDH activity U (mg of protein)−1

ALS/ALDC over-expression (-fold)

BDH over-expression (-fold)

ΔldhA (pEKEx2)*

0.02 ± 0.00

0.66 ± 0.03

–

–

ΔldhA (pEKEx2-als,aldB,butA)*

1.47 ± 0.42

1.18 ± 0.26

61

2

ΔldhA (pEKEx2)

0.04 ± 0.00

0.20 ± 0.03

–

–

ΔldhA (pEKEx2-als,aldB,butA)

0.54 ± 0.09

0.10 ± 0.03

15

–

ΔaceEΔpqoΔldhA (pEKEx2)

0.03 ± 0.01

0.24 ± 0.04

–

–

ΔaceEΔpqoΔldhA (pEKEx2-als,aldB,butA)

0.58 ± 0.12

0.67 ± 0.01

17

3

ΔaceEΔpqoΔldhA (pEKEx2-als,aldB, Ptuf butA)

1.42 ± 0.24

0.53 ± 0.17

31

2

ΔaceEΔpqoΔldhAΔmdh (pEKEx2)

0.06 ± 0.02

0.11 ± 0.04

–

–

ΔaceEΔpqoΔldhAΔmdh (pEKEx2-als,aldB,butA)

0.43 ± 0.04

0.97 ± 0.19

8

8

ΔaceEΔpqoΔldhAΔmdh (pEKEx2-als,aldB, Ptuf butA)

0.47 ± 0.14

1.21 ± 0.01

9

10

  1. Cells were grown for 14 h in 2× TY medium supplemented with acetate, unless stated otherwise
  2. Values shown are averages ± SD of at least three independent experiments and two technical replicates
  3. ALS α-acetolactate synthase, ALDC α-acetolactate decarboxylase, BDH butanediol dehydrogenase
  4. * Cells grown on glucose
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Microbial Cell Factories

ISSN: 1475-2859

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