Table 1 Bacterial strains and plasmids used in this study

E. coli DH5α

Plasmid-free E. coli

Amersham biosciences

C. glutamicum ATCC13032

Wild-type strain

[60]

C. glutamicum ΔldhA

ATCC13032 with deletion of lactate dehydrogenase gene ldhA

This study

C. glutamicum ΔaceEΔpqoΔldhA

ATCC13032 with deletions of the genes for the E1 subunit of pyruvate dehydrogenase complex, the pyruvate:quinone oxidoreductase and the lactate dehydrogenase

[52]

C. glutamicum ΔaceEΔpqo

ΔldhAΔmdh

ATCC13032 with deletions of the genes for the E1 subunit of pyruvate dehydrogenase complex, the pyruvate:quinone oxidoreductase, lactate dehydrogenase and malate dehydrogenase

This study

C. glutamicum ΔaceEΔpqo

ΔldhA (pEKEx2)

C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2

This study

C. glutamicum ΔldhA (pEKEx2)

C. glutamicum ΔldhA harboring plasmid pEKEx2

This study

C. glutamicum ΔaceEΔpqo

ΔldhAΔmdh (pEKEx2)

C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2

This study

C. glutamicum ΔldhA (pEKEx2- als,aldB,butA)

C. glutamicum ΔldhA harboring plasmid pEKEx2 als,aldB,butA

This study

C. glutamicum ΔaceEΔpqo

ΔldhA (pEKEx2-als,aldB,butA)

C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2-als,ald,BbutA

This study

C. glutamicum ΔaceEΔpqo

ΔldhAΔmdh (pEKEx2-als,aldB,butA)

C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2-als,aldB,butA

This study

C. glutamicum ΔaceEΔpqo

ΔldhA (pEKEx2-als,aldB, _Ptuf butA)

C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2-als,aldB, _Ptuf butA

This study

C. glutamicum ΔaceEΔpqo

ΔldhAΔmdh (pEKEx2-als,aldB, _Ptuf butA)

C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2-als,aldB, _Ptuf butA

This study

Plasmids

 pEKEx2

kan^R from pUC4 K; P _trc , lacI, pUC18 mcs, induced by addition of IPTG

[61]

 pEKEx2-als

pEKEx2 with cloned als from L. lactis

This study

 pEKEx2-als,aldB,butA

pEKEx2-als with cloned aldB and butA from L. lactis

This study

 pEKEx2-als,aldB, _Ptuf butA

pEKEx2-als with cloned aldB and P_tuf-butA from L. lactis

This study