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Table 1 Bacterial strains and plasmids used in this study

From: Engineering Corynebacterium glutamicum for the production of 2,3-butanediol

Strains Description Reference
E. coli DH5α Plasmid-free E. coli Amersham biosciences
C. glutamicum ATCC13032 Wild-type strain [60]
C. glutamicum ΔldhA ATCC13032 with deletion of lactate dehydrogenase gene ldhA This study
C. glutamicum ΔaceEΔpqoΔldhA ATCC13032 with deletions of the genes for the E1 subunit of pyruvate dehydrogenase complex, the pyruvate:quinone oxidoreductase and the lactate dehydrogenase [52]
C. glutamicum ΔaceEΔpqo ΔldhAΔmdh ATCC13032 with deletions of the genes for the E1 subunit of pyruvate dehydrogenase complex, the pyruvate:quinone oxidoreductase, lactate dehydrogenase and malate dehydrogenase This study
C. glutamicum ΔaceEΔpqo ΔldhA (pEKEx2) C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2 This study
C. glutamicum ΔldhA (pEKEx2) C. glutamicum ΔldhA harboring plasmid pEKEx2 This study
C. glutamicum ΔaceEΔpqo ΔldhAΔmdh (pEKEx2) C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2 This study
C. glutamicum ΔldhA (pEKEx2- als,aldB,butA) C. glutamicum ΔldhA harboring plasmid pEKEx2 als,aldB,butA This study
C. glutamicum ΔaceEΔpqo ΔldhA (pEKEx2-als,aldB,butA) C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2-als,ald,BbutA This study
C. glutamicum ΔaceEΔpqo ΔldhAΔmdh (pEKEx2-als,aldB,butA) C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2-als,aldB,butA This study
C. glutamicum ΔaceEΔpqo ΔldhA (pEKEx2-als,aldB, Ptuf butA) C. glutamicum ΔaceEΔpqoΔldhA harboring plasmid pEKEx2-als,aldB, Ptuf butA This study
C. glutamicum ΔaceEΔpqo ΔldhAΔmdh (pEKEx2-als,aldB, Ptuf butA) C. glutamicum ΔaceEΔpqoΔldhAΔmdh harboring plasmid pEKEx2-als,aldB, Ptuf butA This study
Plasmids
 pEKEx2 kanR from pUC4 K; P trc , lacI, pUC18 mcs, induced by addition of IPTG [61]
 pEKEx2-als pEKEx2 with cloned als from L. lactis This study
 pEKEx2-als,aldB,butA pEKEx2-als with cloned aldB and butA from L. lactis This study
 pEKEx2-als,aldB, Ptuf butA pEKEx2-als with cloned aldB and Ptuf-butA from L. lactis This study
  1. als α-acetolactate synthase gene, aldB α-acetolactate decarboxylase gene, butA butanediol dehydrogenase gene, P tuf a 185 bp region upstream of tuf gene of C. glutamicum, kan R kanamycin resistance, P trc trc promoter, lacI lac repressor gene