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Fig. 12 | Microbial Cell Factories

Fig. 12

From: Mammalian prion protein (PrP) forms conformationally different amyloid intracellular aggregates in bacteria

Fig. 12

Cytotoxicity of the PK-resistant core of PrP IBs visualized by confocal microscopy. Confocal fluorescence microscopy images of SH-SY5Y cells stained with SYBR green or propidium iodide (PI). Cells were treated with PK-derived fragments of PrPWT IBs (panels cj), or PrP90−231 IBs (k, l). All samples were incubated with PK for 1 h at 37 °C and PK was inactivated before applying to cells. Inactivated PK in PBS was used as control (a, b). Representative images of two independent experiments done in duplicate are shown. Further details are described in the “Methods

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