Fig. 11

PK proteolysis of PrPs IBs. Tricine SDS-PAGE analysis of a PrP^WT IBs and b PrP^90–231 IBs after incubation with 2.5 µg/mL PK at 37 °C for the indicated times. The smaller fragments remaining after PK proteolysis indicate a resistant core of these PrP IBs. The molecular weight ladder (MW) is placed offset for clarity. The arrows indicate C-terminal PrP fragments