Fig. 10

Aggregation kinetics of PrP^WT. The aggregation reactions of 0.5 mg/mL of purified and refolded rPrP^WT were carried out under constant agitation at 600 RPM and 37 °C. In vitro preformed fibrils (2 %) or PrPs IBs (final OD₃₅₀ = 0.1) were used for seeding and cross-seeding assays. PrP fibrilization (black line) as a function of time, exhibits a typical nucleation-elongation profile. The lag phase is reduced in the presence of pre-aggregated homologous protein, either PrP^WT fibrils (blue line) or PrP^WT IBs (red line). Cross-seeding with PrP^90–231 IBs (green line) did not affect the fibrilization extent and kinetics