Lactobacillus plantarum displaying CCL3 chemokine in fusion with HIV-1 Gag derived antigen causes increased recruitment of T cells

Table 2 Primers used in this study

Primer	Sequence
1568F	GGAGTATGATTCATATGAAATTGTTTAAGAAAATTACGAT^a
1568R	CGTATGGGGCGTCGACCGCTGCATAAATTTGCTTAGCAAC^a
cclF	TGCTTCATCAGTCGACGCCCCATACGGTGCT^b
cclR	GTTCAGTGACACGCGTGTTCTTGACTTCTTGACTCGCTTGT^b

^aUnderlining indicates 15-bp extensions that are complementary to the ends of the NdeI/SalI-digested pLp_1261CCL3Gag vector. Overlapping sequences are necessary when using In-fusion cloning
^bUnderlining indicates 15-bp extensions that are complementary to the ends of the SalI/MluI-digested pLp_0373sOFAcwa2 vector

ISSN: 1475-2859