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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Lactobacillus plantarum displaying CCL3 chemokine in fusion with HIV-1 Gag derived antigen causes increased recruitment of T cells

Fig. 4

Flow cytometry (a) and microscopy (b) analysis of surface localization of CCL3Gag. L. plantarum cells harboring plasmids designed for N- or C-terminal anchoring of CCL3Gag were probed with goat anti-CCL3 polyclonal antibody and, subsequently, Alexa Fluor®488-conjugated rabbit anti-goat IgG antibodies. L. plantarum harboring pEV without the ccl3gag gene fragment was used as a negative control and is shown in all five histograms in panel a (black line). The data are presented as one representative experiment. Each experiment was performed at least three independent times and gave the similar results

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Microbial Cell Factories

ISSN: 1475-2859

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