Table 3 Strains and plasmids used in this study
Name | Description | Sources |
|---|---|---|
Strains | ||
S. ansochromogenes 7100 | Wild-type strain | [27] |
ΔwblA | The ORF of whlA consists of 339 bp, and 230 bp of them was replaced by kanamycin resistance gene (neo) | This study |
ΔwblA/pSET152::wblA | The complemented strain of ΔwblA | This study |
Escherichia coli JM109 | recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, Δ(lac-proAB)/F’ [traD36, proAB + lacIq, lacZΔM15] | Invitrogen |
Escherichia coli ET12567/pUZ8002 | dam dcm hsdS cat tet/pUZ8002 | [31] |
Staphylococcus aureus CGMCC1.89 | Indicator strain for bioassays | CGMCC |
Bacillus subtilis CGMCC1.1630 | Indicator strain for bioassays | CGMCC |
Bacillus cereus CGMCC1.1626 | Indicator strain for bioassays | CGMCC |
Candida albicans CGMCC2.4159 | Indicator strain for bioassays | CGMCC |
Alternaria longipes CGMCC3.2946 | Indicator strain for bioassays | CGMCC |
Plasmids | ||
pwblA-DM | Plasmid used for the construction of ΔwblA | This study |
pSET152:: wblA | pSET152 containing the intact wblA with its putative promoter | This study |
pSET152 | Integrative vector | [32] |
pKC1139 | E. coli-Streptomyces shuttle vector | [28] |
pBluescript KS+ | Routine cloning and subcloning vector | Stratagene |