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Table 3 Strains and plasmids used in this study

From: Identification of novel tylosin analogues generated by a wblA disruption mutant of Streptomyces ansochromogenes

Name Description Sources
Strains
 S. ansochromogenes 7100 Wild-type strain [27]
 ΔwblA The ORF of whlA consists of 339 bp, and 230 bp of them was replaced by kanamycin resistance gene (neo) This study
 ΔwblA/pSET152::wblA The complemented strain of ΔwblA This study
 Escherichia coli JM109 recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, Δ(lac-proAB)/F’ [traD36, proAB + lacIq, lacZΔM15] Invitrogen
 Escherichia coli ET12567/pUZ8002 dam dcm hsdS cat tet/pUZ8002 [31]
 Staphylococcus aureus CGMCC1.89 Indicator strain for bioassays CGMCC
 Bacillus subtilis CGMCC1.1630 Indicator strain for bioassays CGMCC
 Bacillus cereus CGMCC1.1626 Indicator strain for bioassays CGMCC
 Candida albicans CGMCC2.4159 Indicator strain for bioassays CGMCC
 Alternaria longipes CGMCC3.2946 Indicator strain for bioassays CGMCC
Plasmids
 pwblA-DM Plasmid used for the construction of ΔwblA This study
 pSET152:: wblA pSET152 containing the intact wblA with its putative promoter This study
 pSET152 Integrative vector [32]
 pKC1139 E. coli-Streptomyces shuttle vector [28]
 pBluescript KS+ Routine cloning and subcloning vector Stratagene
  1. CGMCC China General Microbiological Culture Collection Center