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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Italian legumes: effect of sourdough fermentation on lunasin-like polypeptides

Fig. 3

Expression of the filaggrin (FLG) gene in Caco-2 cells. The expression of the FLG gene in human colon adenocarcinoma cells (Caco-2) was determined using RT-PCR. Caco-2 cells were treated at 37 °C for 4 (A), 8 (B) and 24 h (C) with basal medium containing 1.0 mg/ml of the freeze-dried water/salt soluble extracts obtained from control doughs (D), without bacterial inoculum, and sourdoughs (S), started with selected lactic acid bacteria, made with FL, CV, CC, LN, and PS legume flours. Data are the mean ± SD of three separate experiments, performed in triplicate. ac Columns with different superscript letters differ significantly (P < 0.05)

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