Table 1 Oligonucleotides used

DAO1U1

5′-CACTTTGCTTGTCGAGGACCGTC-3′

5′RACE

DAO1L1

5′-ACGACCAGGTGGCGAAGTGATCT-3′

3′RACE

Rt332f

5′-GCTTGTACTGCTCGAACGAC-3′

cDNA

Rt333r

5′-CTGGTGAAATGCCCCAATAC-3′

cDNA

Rt290Sf

5′-TTTactagtCTTCCCGGTCTCGTATCGAG-3′ (SpeI)

P _DAO1-in1 2.2 kb

Rt315S

5′-TTTactagtACTCCGCAATCTGCAGAGAC-3′ (SpeI)

P _DAO1m2-in1 1.7 kb

Rt314S

5′-TTTactagtCATGGTCTGATCGCTTGTGTG-3′ (SpeI)

P _DAO1m3-in1 1.2 kb

Rt120S

5′-TTTactagtGTGGCAGGTGTGCGTG-3′ (SpeI)

P _DAO1m4-in1 1.0 kb

Rt313S

5′-TTTactagtCGTTCGTGGGCTCAAGGAAG-3′ (SpeI)

P _DAO1m5-in1 0.7 kb

Rt117S

5′-TTTactagtCGACGACGGGAAGCTTCG-3′ (SpeI)

P _DAO1m6-in1 0.4 kb

Rt287Nr

5′-TTTccatggCAATCACTGTATAATCAAGAGCTG-3′ (NcoI)

P _DAO1-in1 reverse

Rt309Nr

5′-TTTccatggCGTCGTTCGAGCAG-3′ (SpeI)

P _DAO1 reverse

Rt311

5′-GAAGCTTCGGCACGAGCATG-3′

P _DAO1m5-in1m1

Rt312

5′-ACAGTCATGCTCGTGCCGAAGCTTCGCAACCGCTCATCAGTACAC-3′

P _DAO1m5-in1m1

SFGFPSEQ

5′-GGACAAACCACAACTAGAATGCAG

P _DAO1m5-in1m1

35STer

5′-AAAGCATGCTAATTCGGGGGATCTGGAT

P _DAO1m5-in1m1

Rt288f

5′-GTAGGTTACGCCGATCGAGTTG-3′

DAO1 Probe

Rt289r

5′-GCTCGACCAACTGCTCTCTTTC-3′

DAO1 Probe

Rt327r

5′-GGCGTCGTTCGAGCAGTAC-3′

P _DAO1m5-in1m2

Rt328f

5′-CTGCTTGTACTGCTCGAACGACGCCATCCATTCACAGAAGCGCGTCGT-3′

P _DAO1m5-in1m2

Rt329r

5′-GACGCACCGCCTGATCCGAG-3′

P _DAO1m5-in1m2

P _DAO1m5-in1m3

Rt330f

5′-TTGTCCTCGGATCAGGCGGTGCGTCTTTAAATATAATAAAAAAAAAAGACAGTTCTCGAGGAGGAGTAC-3′

P _DAO1m5-in1m2

Rt331f

5′-TTGTCCTCGGATCAGGCGGTGCGTC(24 mer deletion)CAGTTCTCGAGGAGGAGTAC-3′

P _DAO1m5-in1m3

Rt334f

5′-TTGTCCTCGGATCAGGCGGTGCGTCTTTCAATCTCCTCCCCACACCCGACAGTTCTCGAGGAGGAGTAC-3′

P _DAO1m5-in1m4

Rt335f

5′-TTGTCCTCGGATCAGGCGGTGCGTCTTTCCCTCTAATCCCCACACCCGACAGTTCTCGAGGAGGAGTAC-3′

P _DAO1m5-in1m5

Rt336f

5′-TTGTCCTCGGATCAGGCGGTGCGTCTTTCCCTCTCCTCAACACACCCGACAGTTCTCGAGGAGGAGTAC-3′

P _DAO1m5-in1m6

Rt337f

5′-TTGTCCTCGGATCAGGCGGTGCGTCTTTCCCTCTCCTCCCCGCACCCGACAGTTCTCGAGGAGGAGTAC-3′

P _DAO1m5-in1m7

Rt338f

5′-TTGTCCTCGGATCAGGCGGTGCGTCTTTCCCTCTCCTCCCCACACAAGACAGTTCTCGAGGAGGAGTAC-3′

P _DAO1m5-in1m8

LUC2U

5′-GAAGTACTCGGCGTAGGTG-3′

In RtLUC2, for amplification of P _{DAO1m1-in1m1~} P _DAO1m5-in1m8

DAO1f

5′-CTTCGTGCTAACCAAGCTCGT-3′

Probe and colony PCR of DAO1

DAO1r

5′-GTCTCAGGGTTGACGGACAAG-3′

Probe and colony PCR of DAO1

qDAO1f

5′-TCAAACCGTCCTCGTCAAGTC-3′

qPCR of DAO1

qDAO1r

5′-GTTGACGGACAAGTCCCAATC-3′

qPCR of DAO1

qACT1f

5′-TACCCAACTTGTCCCAACCTG-3′

qPCR of ACT1, reference gene

qACT1r

5′-CTCGTCTCCATCACCATCCTC-3′

qPCR of ACT1, reference gene

DAO1L-Sf

5′-AAAgagctcGACTCGTTGGGCAAAGTGAAG-3′ (SacI)

Deletion of DAO1

DAO1L-Br

5′-AAAggatccGGAAGCGCACAAAGTCAATTC-3′ (BamHI)

Deletion of DAO1

DAO1R-Hf

5′-TTTaagcttCAAAGGAGAAGGAGGTGACA-3′ (HindIII)

Deletion of DAO1

DAO1R-Str

5′-TTTaggcctGTCTATTTGCGGTGGAATGGA-3′ (StuI)

Deletion of DAO1