Fig. 9

Effects of DAO1 gene deletion. a Southern blot analysis of DAO1 knockout mutants. Genomic DNA (2 µg) was digested with PstI and hybridized against the digoxigenin-labeled probe of DAO1R (Probe 2 in Fig. 2a). b Growth of DAO1 null mutant and WT in YNB medium with d-alanine and l-alanine as the sole carbon source. c Response of 2.2 kb P _DAO1in1 in WT and DAO1 knockout mutant (Δdao1e) in MinABs supplemented with glucose (10 g/L), ammonium sulfate (70 mM), l-alanine (l-ala, 70 mM). (+) indicates 70 mM d-alanine while other concentrations used are marked with +10 (mM) and +1 (mM), respectively. d Time course of 2.2 kb P _DAO1-in1 activity in WT and Δdao1e strains. Strains were cultured in MinABs medium supplemented with various concentrations of d-alanine indicated