Fig. 8

Effects of carbon and nitrogen sources on DAO1 promoter activity. All assays were done in triplicates with a luciferase reporter strain containing the 2.2-kb P _DAO1-in1 fused to RtLUC2 :T _35S and knocked in at CAR2 locus. a Effect of carbon and nitrogen sources. Cells were cultured for 21 h in MinABs supplemented with the designated carbon and nitrogen sources. G glucose (10 g/L), AS ammonium sulfate (70 mM), d -ala d-alanine (70 mM), l -ala l-alanine (70 mM). b Effects of different concentrations of d-alanine. 10 g/L glucose and different concentrations of d-alanine were supplemented to the basal medium MinABs. c Effects of different concentrations of glucose. 70 mM d-alanine and different concentration of d-alanine were supplemented to basal medium MinABs. d Effects of different concentrations of ammonium sulfate. 10 g/L glucose, 70 mM d-alanine and different concentrations of ammonium sulfate were supplemented to basal medium MinABs. e Time course of promoter activities. 70 mM d-alanine was used as the sole carbon and nitrogen source. f Effects of different d-amino acids as the inducer. d and l represents d- and l-alanine, respectively