Fig. 6From: Engineering an efficient and tight d-amino acid-inducible gene expression system in Rhodosporidium/Rhodotorula speciesFunctional dissection of DAO1 promoter. a Schematic diagram of serial deletions of DAO1 promoter knocked in at CAR2 locus. b Effect of motif 1 and intron 1. Promoter activities were assayed with luciferase kit using cells cultured for 21 h in MinABs medium supplemented with d-alanine or l-alanine. c Relative promoter activities (RPA) of various deletions. GPD1 promoter refers to the 795-bp promoter of glyceraldehyde-3-phosphate dehydrogenase gene. P-less promoter-less background controlBack to article page