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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Engineering an efficient and tight d-amino acid-inducible gene expression system in Rhodosporidium/Rhodotorula species

Fig. 5

RtLUC2 reporter system. a Schematic diagram of DAO1 gene structure and the T-DNA regions of reporter plasmid pKCL2 and pKCLDx. RB and LB: right and left borders Agrobacterium tumefaceins T-DNA; CAR2L and CAR2R: left and right homology arms of CAR2 locus that were used for locus-directed integration of RtLUC2 reporter constructs; P GPD1-3 : GPD1 promoter of Rhodotorula graminis WP1; hpt-3: codon-optimized hygromycin resistance gene; RtLUC2: codon-optimized Luciferase gene; loxP-RE and loxP-LE: mutant recognition sites for Cre reccombinase [41], T 35S : transcriptional terminator of Cauliflower mosaic virus gene 35S; DAOx indicates various DAO1 promoter fragments in the reporters; b T-DNA regions of binary vector pRHE33. c Important features of the DAO1 mRNA transcription. tsp transcriptional start point; Terminal sequence of P DAO1-in1 promoters are shown in the box. d Typical colony color phenotypes of pKCL2/pKCLDx transformants

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