Fig. 5
From: High-level secretion of native recombinant human calreticulin in yeast
Partial digestion of native and recombinant human CRTs with trypsin. M unstained protein ladder (ThermoScientific, cat. no. 26614). Undigested CRTs are loaded to the left, CRTs digested with trypsin in the presence of 3 mM CaCl2—in the middle, whereas CRTs digested with trypsin after addition of EGTA to chelate calcium ions—in the right gel lanes. Source of protein is indicated as described in Fig. 2 legend