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Table 1 Synechocystis strains used in this study

From: Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production

Strain Plasmid Genome modification Notes
JA01 pJA2-P psbA2 phaJ ter adhE2 None Butanol biosynthesis genes on replicating plasmid
JA02 pJA2-P psbA2 phaJ ter adhE2 ΔphaEC::SpR PHB synthase deletion eliminates PHB production
JA03 pJA2-P psbA2 phaJ ter adhE2 ΔphaEC::SpR, ΔNSI::P trc phaAB CmR Overexpression of the native phaA and phaB genes
JA04 pJA8-P trc phaJ ter adhE2 ΔphaEC::SpR P psbA2 replaced with the stronger trc promoter
JA05 pJA8-P trc phaJ ter adhE2 ΔphaEC::SpR, ΔNSI::P trc phaAB CmR  
JA06 None ΔNSI::P trc xfpk CmR Expression of a phosphoketolase
JA07 pJA8-P trc phaJ ter adhE2 ΔphaEC::SpR ΔNSI::P trc xfpk CmR