Table 1 Synechocystis strains used in this study
From: Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production
Strain | Plasmid | Genome modification | Notes |
|---|---|---|---|
JA01 | pJA2-P psbA2 phaJ ter adhE2 | None | Butanol biosynthesis genes on replicating plasmid |
JA02 | pJA2-P psbA2 phaJ ter adhE2 | ΔphaEC::SpR | PHB synthase deletion eliminates PHB production |
JA03 | pJA2-P psbA2 phaJ ter adhE2 | ΔphaEC::SpR, ΔNSI::P trc phaAB CmR | Overexpression of the native phaA and phaB genes |
JA04 | pJA8-P trc phaJ ter adhE2 | ΔphaEC::SpR | P psbA2 replaced with the stronger trc promoter |
JA05 | pJA8-P trc phaJ ter adhE2 | ΔphaEC::SpR, ΔNSI::P trc phaAB CmR | |
JA06 | None | ΔNSI::P trc xfpk CmR | Expression of a phosphoketolase |
JA07 | pJA8-P trc phaJ ter adhE2 | ΔphaEC::SpR ΔNSI::P trc xfpk CmR |